很棒的肉汤基础Autoinducible w / o金属500克/瓶

很棒的肉汤基础Autoinducible w / o Metalsis媒介支持细胞密度高,在制定最优增长E。杆菌、保持增长的对数期很长时间了。因此,它得到更多的重组蛋白和血浆的DNA。在某些情况下,它的替代品磅肉汤(Lennox)(猫。1231)用于基因研究。自动感应媒体首先制定并由w .研究人IPTG-inducible表达菌株生长。自动感应媒体的原则是基于碳源代谢不同的媒介促进高密度细胞生长和自动从lac推动者诱导蛋白表达。自动感应媒体含有葡萄糖和乳糖作为碳源。有限浓度的葡萄糖代谢中优先增长,从而防止吸收乳糖葡萄糖耗尽之前,通常在日志中后期阶段。随着葡萄糖耗尽,乳糖可以吸收和转化酶aÿ-galactosidase诱导物的异乳糖。异乳糖引起的释放阻遏物从其特定的DNA结合位点,从而诱导表达lacUV5 T7 RNA聚合酶的启动子和打破T7lac推动者,并允许表达目标蛋白质的T7 RNA聚合酶。自动感应媒体高密度细胞生长是紧随其后的是一个自发诱导蛋白表达。 There is no need to monitor the cell density and there is no conventional induction with IPTG. Parallel growth of many non-induced or auto-induced cultures is feasible because cultures are simply inoculated and grown to saturation. This is a great convenience and simplifies manual or automated induction and analysis of multiple clones compared to conventional IPTG induction, which requires monitoring growth of each culture and adding inducer at the proper stage of growth. For higher saturation density use the Super Growth Autoinducible Medium w/o Trace Element (Cat. 2095). Tryptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group. Potassium phosphates act as a buffer system to prevent cell death. If desired, add a concentration of 0.015 g/l of Trace Elements (Cat. 2109) to supply all of the specific requirements for the bacteria.