肠球菌选择性肉汤(ENTEROCCOSEL肉汤)500克/瓶

肠球菌选择性肉汤(Emterococcosel肉汤)是一个敏感的分离富集培养基enterococci从包含许多其他植物标本。许多生物如腐生的脑膜炎,葡萄球菌、嗜血杆菌,non-hemolytic链球菌,和一定数量的肠杆菌科抑制全部或部分。酪蛋白和大豆蛋白胨为增长提供必需营养素。葡萄糖是可发酵碳水化合物的能量来源。氯化钠维持渗透平衡。柠檬酸钠提供了额外的碳。叠氮化钠是一种抑制剂。亚硫酸钠时减少产生硫化氢。l -胱氨酸降低氧化还原电位通过消除氧气保持低啊。结晶紫是一种酸碱指示剂。 Clinical material is inoculated into this selective medium and tubes are incubated at 35 °C for 18-24 hours in a normal atmosphere. The growth of streptococci can be determined by the formation of a granular precipitate at the bottom of the tube, with the liquid above being clean or slightly turbid. At this point, perform a Gram stain and restreak on Trypticasein Soy Agar (Cat. 1068) blood plates or Blood Agar Base (Cat. 1108) to determine the type of hemolysis and to purify the culture. The presence of variable length chains of gram-positive cocci inhibited by bacitracin in a low concentration, catalase negative and insoluble in bile or bile salts, constitute a valid presumptive identification of Group A beta-hemolytic streptococci. The definitive identification of the streptococci groups can be made by performing other biochemical tests such as esculin hydrolysis, pyruvate hydrolysis, etc. Also, serological typing, using Lancefield antisera methods, or more conveniently, the techniques of co-agglutination of Edwards and Larson can be performed.