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琼脂布鲁氏菌富含营养物质和生长因子,非常适合培养和分离挑剔的微生物。该方法用于从临床样品和食品中被腐生菌和共生菌污染的各种标本中成功分离布鲁氏菌。这种培养基也用于产生梭菌毒素。它也可用于分离人类和动物来源的许多厌氧菌。食品样品可直接接种在琼脂布鲁氏菌培养皿上,而临床标本则更方便采用悬浮液或无菌生理盐水浸泡。肉蛋白胨和酪蛋白蛋白胨提供生长所必需的氮、维生素、矿物质和氨基酸。酵母提取物是维生素的来源,尤其是维生素b族。亚硫酸氢钠为还原剂;氯化钠为运输和渗透平衡提供必需的电解质。葡萄糖是可发酵的碳水化合物,提供碳和能量。 The addition of blood provides extra growth factors for fastidious microorganisms. Bacteriological agar is the solidifying agent. The addition of the Brucella Supplement (Cat. 6017) enhances the mediums selectivity for the growth of Brucella. For a better growth, Polyenrichment Supplement (Cat. 6011) and Polyenrichment CC Supplement (Cat. 6071) may be added if required. Brucella species are level 3 pathogens and cause brucellosis, a zoonotic disease. It is usually transmitted through milk, dairy products, meat and direct contact with infected animals. Note: To obtain an excellent medium for anaerobes, add 5 mg/ml of hemin and 10 µg/ml of vitamin K1 (fitomenadione) to the basal medium. Inoculate and incubate in anaerobic conditions
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